2'-OMe-ibu-G-CE Phosphoramidite

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10-3120
5'-Dimethoxytrityl-N-isobutyryl-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C45H56N7O9P
M.W.:
869.97
F.W.:
359.24
CAS Number:
150780-67-9
Catalog: Make Selections to Reveal

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

  • Coupling: 6 minute coupling time
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3120-02 0.25 g .25grams 2.87 82.33 49.4 30.88 22.45 16.47 4.12
10-3120-05 0.5 g .5grams 5.75 178.33 107 66.88 48.64 35.67 8.92
10-3120-10 1.0 g 1grams 11.49 369.67 221.8 138.63 100.82 73.93 18.48
10-3120-90 100 µmol .087grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3120-02 0.25 g .25grams 4.29 0.07 79.4 49.63 36.09 4.96
10-3120-05 0.5 g .5grams 8.58 0.07 165.2 103.25 75.09 10.33
10-3120-10 1.0 g 1grams 17.16 0.07 336.8 210.5 153.09 21.05
10-3120-90 100 µmol .087grams 1.5 0.07 23.6 14.75 10.73 1.48