2'-OMe-Ac-C-CE Phosphoramidite

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10-3115
5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C42H52N5O9P
M.W.:
801.88
F.W.:
319.21
CAS Number:
199593-09-4
Catalog: Make Selections to Reveal

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

  • Coupling: 6 minute coupling time
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3115-02 0.25 g .25grams 3.12 90.67 54.4 34 24.73 18.13 4.53
10-3115-05 0.5 g .5grams 6.24 194.67 116.8 73 53.09 38.93 9.73
10-3115-10 1.0 g 1grams 12.47 402.33 241.4 150.88 109.73 80.47 20.12
10-3115-90 100 µmol .08grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3115-02 0.25 g .25grams 4.65 0.07 86.6 54.13 39.36 5.41
10-3115-05 0.5 g .5grams 9.31 0.07 179.8 112.38 81.73 11.24
10-3115-10 1.0 g 1grams 18.61 0.07 365.8 228.63 166.27 22.86
10-3115-90 100 µmol .08grams 1.5 0.07 23.6 14.75 10.73 1.48