2-Aminopurine-TBDMS-CE Phosphoramidite

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10-3070
5'-Dimethoxytrityl-N2-isobutyryl-2-aminopurine riboside, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite
Formula:
C50H68N7O8PSi
M.W.:
954.19
F.W.:
329.21
CAS Number:
151059-65-3
Catalog: Make Selections to Reveal

Description

2-Aminopurine riboside is useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Details

Usage

  • Coupling: 12 minute coupling time recommended. See Technical Bulletin
  • Deprotection: RNA deprotaction using Ammonium Hydroxide:MethylAmine (AMA) 1:1 at 65°C for 10 minutes. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3070-02 0.25 g .25grams 2.62 74 44.4 27.75 20.18 14.8 3.7
10-3070-90 100 µmol .095grams 1 20 12 7.5 5.45 4 1
10-3070-95 50 µmol .048grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3070-02 0.25 g .25grams 3.91 0.07 0 0 14.96 0
10-3070-90 100 µmol .095grams 1.5 0.07 0 0 4.92 0
10-3070-95 50 µmol .048grams 0.75 0.07 0 0 1.79 0