Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.
Coupling: Synthesize oligoribonucleotide using standard TOM RNA chemistry (6 min. coupling time).
Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_4-Thio-U-TOM.pdf).