5-Me-U-CE Phosphoramidite

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10-3050
5'-Dimethoxytrityl-5-Methyl-Uridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C46H63N4O9PSi
M.W.:
875.08
F.W.:
320.19
Catalog: Make Selections to Reveal

Description

Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships.

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: RNA deprotection. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3050-02 0.25 g .25grams 2.86 82 49.2 30.75 22.36 16.4 4.1
10-3050-90 100 µmol .088grams 1 20 12 7.5 5.45 4 1
10-3050-95 50 µmol .044grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3050-02 0.25 g .25grams 4.26 0.07 0 0 16.42 0
10-3050-90 100 µmol .088grams 1.5 0.07 0 0 4.92 0
10-3050-95 50 µmol .044grams 0.75 0.07 0 0 1.79 0