U-TOM-CE Phosphoramidite

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10-3034

5'-Dimethoxytrityl-Uridine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula:
C49H69N4O10PSi
M.W.:
933.17
F.W.:
306.17
Catalog: Make Selections to Reveal

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Usage

Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours

Intellectual Property

TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3034-02 0.25 g 0.25grams 2.68 76 45.6 28.5 20.73 15.2 3.8
10-3034-05 0.5 g 0.5 grams 5.36 165.33 99.2 62 45.09 33.07 8.27
10-3034-10 1.0 g 1grams 10.72 344 206.4 129 93.82 68.8 17.2

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3034-02 0.25 g 0.25grams 4 0.07 0 0 15.33 0
10-3034-05 0.5 g 0.5 grams 8 0.07 0 0 32 0
10-3034-10 1.0 g 1grams 15.99 0.07 0 0 65.29 0