G-TOM-CE Phosphoramidite

default
10-3024
5'-Dimethoxytrityl-N-acetyl-Guanosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C52H72N7O10PSi
M.W.:
1014.24
F.W.:
345.21
Catalog: Make Selections to Reveal

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Usage

  • Coupling: See Technical Bulletin
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TOM_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours

Intellectual Property

TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3024-02 0.25 g .25grams 2.46 68.67 41.2 25.75 18.73 13.73 3.43
10-3024-05 0.5 g .5grams 4.93 151 90.6 56.63 41.18 30.2 7.55
10-3024-10 1.0 g 1grams 9.86 315.33 189.2 118.25 86 63.07 15.77

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3024-02 0.25 g .25grams 3.68 0.07 0 0 14 0
10-3024-05 0.5 g .5grams 7.36 0.07 0 0 29.33 0
10-3024-10 1.0 g 1grams 14.72 0.07 0 0 60 0