iPr-Pac-G-CE Phosphoramidite

default
10-3021
5'-Dimethoxytrityl-N-p-isopropylphenoxyacetyl-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C57H74N7O10PSi
M.W.:
1076.31
F.W.:
345.21
Catalog: Make Selections to Reveal

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certi cate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling ef ciency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minutes. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3021-02 0.25 g .25grams 2.32 64 38.4 24 17.45 12.8 3.2
10-3021-05 0.5 g .5grams 4.65 141.67 85 53.13 38.64 28.33 7.08
10-3021-10 1.0 g 1grams 9.29 296.33 177.8 111.13 80.82 59.27 14.82

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3021-02 0.25 g .25grams 3.47 0.07 0 0 13.13 0
10-3021-05 0.5 g .5grams 6.93 0.07 0 0 27.54 0
10-3021-10 1.0 g 1grams 13.87 0.07 0 0 56.46 0