Pyrrolo-C-TOM-CE Phosphoramidite

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10-3017
5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C52H72N5O9PSi
M.W.:
970.23
F.W.:
343.27
Catalog: Make Selections to Reveal

Description

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days

Intellectual Property

This product is covered by US Patent No.: 7,144,995.|Patented under European patent number 1483280.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3017-02 0.25 g .25grams 2.58 72.67 43.6 27.25 19.82 14.53 3.63
10-3017-90 100 µmol .097grams 1 20 12 7.5 5.45 4 1
10-3017-95 50 µmol .049grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3017-02 0.25 g .25grams 3.85 0.07 0 0 14.71 0
10-3017-90 100 µmol .097grams 1.5 0.07 0 0 4.92 0
10-3017-95 50 µmol .049grams 0.75 0.07 0 0 1.79 0