Ac-C-CE Phosphoramidite

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10-3015
5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C47H64N5O9PSi
M.W.:
902.11
F.W.:
305.18
CAS Number:
121058-88-6
Catalog: Make Selections to Reveal

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3015-02 0.25 g .25grams 2.77 79 47.4 29.63 21.55 15.8 3.95
10-3015-05 0.5 g .5grams 5.54 171.33 102.8 64.25 46.73 34.27 8.57
10-3015-10 1.0 g 1grams 11.09 356.33 213.8 133.63 97.18 71.27 17.82

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3015-02 0.25 g .25grams 4.14 0.07 0 0 15.92 0
10-3015-05 0.5 g .5grams 8.27 0.07 0 0 33.13 0
10-3015-10 1.0 g 1grams 16.54 0.07 0 0 67.58 0