C-TOM-CE Phosphoramidite

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10-3014
5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C51H72N5O10PSi
M.W.:
974.22
F.W.:
305.18
Catalog: Make Selections to Reveal

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Usage

  • Coupling: See Technical Bulletin
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TOM_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours

Intellectual Property

TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3014-02 0.25 g .25grams 2.57 72.33 43.4 27.13 19.73 14.47 3.62
10-3014-05 0.5 g .5grams 5.13 157.67 94.6 59.13 43 31.53 7.88
10-3014-10 1.0 g 1grams 10.26 328.67 197.2 123.25 89.64 65.73 16.43

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3014-02 0.25 g .25grams 3.83 0.07 0 0 14.63 0
10-3014-05 0.5 g .5grams 7.66 0.07 0 0 30.58 0
10-3014-10 1.0 g 1grams 15.32 0.07 0 0 62.5 0