Pac-A-CE Phosphoramidite

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10-3000

5'-Dimethoxytrityl-N-phenoxyacetyl-Adenosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula:

C₅₄H₆₈N₇O₉PSi

M.W.:

1018.23

F.W.:

329.21

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Description

Details

Technical Documents

Dilution/Coupling Data

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certi cate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling ef ciency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in may be substituted with Ac-G (10-3025).

Details

Usage

Coupling: 12 minutes. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: RNA Deprotection-see bulletin

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30°C, dry
Stability	24 hours

Technical Documents

Safety Data Sheet

PROCEDURE FOR THE SYNTHESIS, DEPROTECTION AND ISOLATION OF RNA USING TBDMS-PROTECTED MONOMERS

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3000-02	0.25 g	.25grams	2.46	68.67	41.2	25.75	18.73	13.73	3.43
10-3000-05	0.5 g	.5grams	4.91	150.33	90.2	56.38	41	30.07	7.52
10-3000-10	1.0 g	1grams	9.82	314	188.4	117.75	85.64	62.8	15.7