iPr-Pac-dG-CE Phosphoramidite

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10-1621

5'-Dimethoxytrityl-N-p-isopropyl-phenoxyacetyl-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula:
C51H60N7O9P
M.W.:
946.05
F.W.:
329.21
CAS Number:
150065-82-0
Catalog: Make Selections to Reveal

Description

An alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.

We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix. Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
  • Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1621-02 0.25 g .25grams 2.64 74.67 44.8 28 20.36 14.93 3.73
10-1621-05 0.5 g .5grams 5.29 163 97.8 61.13 44.45 32.6 8.15
10-1621-10 1.0 g 1grams 10.57 339 203.4 127.13 92.45 67.8 16.95

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1621-02 0.25 g .25grams 3.94 0.07 72.4 45.25 32.91 4.53
10-1621-05 0.5 g .5grams 7.89 0.07 151.4 94.63 68.82 9.46
10-1621-10 1.0 g 1grams 15.78 0.07 309.2 193.25 140.55 19.33