dC-5'-CE Phosphoramidite

default
10-0101
3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C46H52N5O8P
M.W.:
833.93
F.W.:
289.18
CAS Number:
140712-83-0
Catalog: Make Selections to Reveal

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in about 5% N4-methyl-dC side product at the modification site.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability Similar to dA,C,G,T-CE Phosphoramidites


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-0101-02 0.25 g .25grams 3.05 86.67 52 32.5 23.64 17.33 4.33
10-0101-05 0.5 g .5grams 6 186.67 112 70 50.91 37.33 9.33
10-0101-10 1.0 g 1grams 11.99 386.33 231.8 144.88 105.36 77.27 19.32

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-0101-02 0.25 g .25grams 4.47 0.07 83.55 51.88 37.73 5.19
10-0101-05 0.5 g .5grams 8.95 0.07 172.6 107.88 78.45 10.79
10-0101-10 1.0 g 1grams 17.9 0.07 351.6 219.75 159.82 21.98