Bz-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-Adenosine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:

C₅₃H₆₆N₇O₈PSi

M.W.:

988.21

F.W.:

329.21

CAS Number:

104992-55-4

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Description

Details

Technical Documents

Dilution/Coupling Data

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

Coupling: 12 minute coupling time recommended
Deprotection: RNA Deprotection-see bulletin

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30°C, dry
Stability	24 hours

Technical Documents

Safety Data Sheet

TBDMS-Protected RNA Guide

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 9.12: Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers

Glen Report 4-12: RNA Synthesis - Options for 2'-0H Protection

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3003-02	0.25 g	.25grams	2.53	71	42.6	26.63	19.36	14.2	3.55
10-3003-05	0.5 g	.5grams	5.06	155.33	93.2	58.25	42.36	31.07	7.77
10-3003-10	1.0 g	1grams	10.12	324	194.4	121.5	88.36	64.8	16.2

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3003-02	0.25 g	.25grams	3.78	0.07	69	43	14.42	4
10-3003-05	0.5 g	.5grams	7.55	0.07	144	90	30.13	9
10-3003-10	1.0 g	1grams	15.1	0.07	295	184	61.58	18