Bz-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-Adenosine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C53H66N7O8PSi
M.W.:
988.21
F.W.:
329.21
CAS Number:
104992-55-4
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3003-02 0.25 g .25grams 2.53 71 42.6 26.63 19.36 14.2 3.55
10-3003-05 0.5 g .5grams 5.06 155.33 93.2 58.25 42.36 31.07 7.77
10-3003-10 1.0 g 1grams 10.12 324 194.4 121.5 88.36 64.8 16.2

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3003-02 0.25 g .25grams 3.78 0.07 69 43 14.42 4
10-3003-05 0.5 g .5grams 7.55 0.07 144 90 30.13 9
10-3003-10 1.0 g 1grams 15.1 0.07 295 184 61.58 18