Ultramild RNA Synthesis

RNA contains an astonishing variety of minor bases in addition to the four canonical bases. As research into the function of these naturally-occurring minor bases develops, there will be a need to synthesize oligonucleotides containing these minor bases. Each minor base will likely lead to a mini research project to identify the protection scheme required to prepare the phosphoramidite but, especially, to efine the conditions required for deprotection. As this research progresses, there is no doubt that the use of UltraMild RNA synthesis will be required to allow the deprotected oligos to be isolated in the required purity.