RNA contains an astonishing variety of minor bases in addition to the four canonical bases. As research into the function of these naturally-occurring minor bases develops, there will be a need to synthesize oligonucleotides containing these minor bases. Each minor base will likely lead to a mini research project to identify the protection scheme required to prepare the phosphoramidite but, especially, to efine the conditions required for deprotection. As this research progresses, there is no doubt that the use of UltraMild RNA synthesis will be required to allow the deprotected oligos to be isolated in the required purity.
An UltraMild set of CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis is available for situations where sensitive bases are in use.
The use of UltraMild monomers in oligonucleotide synthesis has allowed very sensitive dyes like TAMRA, HEX and Cy5 to be used virtually routinely.
An alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels. Glen Research offers supports to support UltraMild RNA synthesis.
UltraMild DNA synthesis reagents are available for use when DNA bases and labels are not compatible with ammonium hydroxide deprotection.