TOM-Protected RNA Synthesis

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection.  High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers.  Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature.  A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration.  This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group.  These features allow the TOM-Protected monomers to produce longer oligonucleotides.  TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.

INTELLECTUAL PROPERTY

TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN.  RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084.

TOM-Protecting-Group is a trademark of QIAGEN.  


Explore TOM-Protected RNA Synthesis

Glen Research offers minor RNA phosphoramidites with TOM TBDMS protecting groups.

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection.

Glen Research offers supports for TOM RNA synthesis..

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