TOM-Protected RNA Synthesis

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection.  High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers.  Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature.  A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration.  This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group.  These features allow the TOM-Protected monomers to produce longer oligonucleotides.  TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.

 

INTELLECTUAL PROPERTY

TOM-Protecting-Group is a trademark of QIAGEN.  


Explore TOM-protected phosphoramidites and supports for RNA synthesis

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection.

Glen Research offers Supports with TOM protecting groups.

Glen Research offers minor RNA phosphoramidites with TOM TBDMS protecting groups.

Amino-Modifier C6-U has been added to the growing family of sequence modifiers and we envisage applications in RNA structural studies as well as for labeling siRNA to probe uptake and cellular distribution.