CleanAmp™ Primers offer an alternative to other Hot Start technologies and allow greater control of primer hybridization and extension during PCR. It has been demonstrated that CleanAmp Primers outperform other technologies in multiple applications. Indeed, over a broad range of applications, CleanAmp Primers reduce or eliminate off-target amplification. Greater amplicon yield is also achieved, due to improvement in specificity and sensitivity. By using either the slow-releasing Precision primers with two CleanAmp phosphotriester linkages or the faster-releasing Turbo Primers with a single CleanAmp phosphotriester linkage, the rate of formation of unmodified primer can be controlled to suit reaction needs. A table to aid in the selection of Turbo and Precision Primers for specific applications is shown below.
|Turbo Primers||Precision Primers|
|Fast cycling||Standard cycling|
|Multiplex PCR||One-step reverse-transcription PCR|
|Improves amplicon yield||Improved specificity and limit of detection|
|Reduces mis-priming/ primer dimer formation||Greatest reduction in mis-priming/primer dimer formation|
Synthesis of CleanAmp Primers requires the use of UltraMild Chemistry.
CleanAmp™ Primers and monomers are available from TriLink BioTechnologies.hot