COT Serinol Phosphoramidite

Bright, long-lasting and non-phototoxic organic fluorophores are essential for the continued optimization of a diverse range of imaging applications. However, all currently available technologies remain susceptible to undesirable transitions to dark states. Dark states arise from non-fluorescent triplet electronic configurations from which the rate of return to the ground state is slow. When in the triplet state, the fluorophore is susceptible to photobleaching and fluorescence applications are compromised by unpredictably reducing the signal-to-noise ratio (SNR), as well as limiting the total duration of time over which information can be gathered. The direct conjugation of small-molecule protective agents (PAs) has enabled significant improvements through intra-molecular triplet quenching. Through a partnership with Lumidyne Technologies, Glen Research has created a novel PA-linked phosphoramidite using cyclooctatetraene (COT). COT Serinol Phosphoramidite provides a means to improve the photostability of virtually any fluorophore in a modular fashion. Our spectrofluorometric studies show that the presence of COT limited the amount of photobleaching of an oligo containing the cyanine 5 dye.