Br-U-CE Phosphoramidite

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10-3090
5'-Dimethoxytrityl-5-bromo-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C45H60N4O9BrPSi
M.W.:
939.96
F.W.:
385.06
Catalog Number: Select Pack Size and Format to Reveal

Description

5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments.

Details

Usage

  • Coupling: 12 minutes
  • Deprotection: Ammonium hydroxide/Ethanol 3:1 (v/v) at room temperature as required by the nucleobases. Complete the 2' deprotection using standard RNA protocols. See http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3090-02 0.25 g .25grams 2.66 75.33 45.2 28.25 20.55 15.07 3.77
10-3090-90 100 µmol .094grams 1 20 12 7.5 5.45 4 1
10-3090-95 50 µmol .047grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3090-02 0.25 g .25grams 3.97 0.07 0 0 15.21 0
10-3090-90 100 µmol .094grams 1.5 0.07 0 0 4.92 0
10-3090-95 50 µmol .047grams 0.75 0.07 0 0 1.79 0