BiotinTEG Phosphoramidite

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10-1955

1-Dimethoxytrityloxy-3-O-(N-biotinyl-3-aminopropyl)-triethyleneglycolyl-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C52H76N5O11PS
M.W.:
1010.24
F.W.:
569.61
CAS Number:
198080-44-3
Catalog Number: Select Pack Size and Format to Reveal

Description

Glen Research biotin phosphoramidites for direct labelling of synthetic oligonucleotides exhibit the following features:

  1. All are soluble in acetonitrile at concentrations useful for DNA synthesis.
  2. All include a DMT group for cartridge puri cations which is essential for the preparation of biotinylated PCR primers because of the potential for cross contamination in HPLC puri cations.
  3. For the development of diagnostic probes, biotin phosphoramidite is capable of branching to allow multiple biotins to be introduced at the 3’- or 5’-terminus. BiotinTEG Phosphoramidite contains a 15 atom mixed polarity spacer arm based on a triethylene glycol.

Details

Usage

  • Coupling: 12-15 minute coupling time. To maintain label yield, carry out the synthesis DMT-on.
  • Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group.  Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with ACN. At this point, the DMT group may be safely removed without loss of label during deprotection.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1955-02 0.25 g .25grams 2.47 69 41.4 25.88 18.82 13.8 3.45
10-1955-90 100 µmol .101grams 1 20 12 7.5 5.45 4 1
10-1955-95 50 µmol .051grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1955-02 0.25 g .25grams 3.69 0.07 67.4 42.13 30.64 4.21
10-1955-90 100 µmol .101grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1955-95 50 µmol .051grams 0.75 0.07 8.6 5.38 3.91 0.54


Product FAQs

  • What absorbance does biotin have at 260nm? The HPLC trace shows absorbance at 254nm?
  • Do you have a biotin phosphoramidite containing a disulfide linker which can be cleaved later with DTT to release the DNA from a streptavidin support?
  • How can I tell if the biotinylated oligonucleotide I have made really does contain biotin?
  • Can oligonucleotides modified at the 5'-terminus with, for example, biotin be phosphorylated with kinase?