Biotin is a popular oligonucleotide modification and is used in a wide range of applications, including enrichment, detection, and immobilization assays. 5’-Biotin II consists of an ethylene glycol linker, an alternative structure to our traditional version. 5’-Biotin II phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques.
Details
Usage
Coupling: 2 minute coupling time recommended
Deprotection: 5'-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step. If the final DMT-group is left on to aid in cartridge purification, we recommend that the oligo is left in contact with the TFA solution for 10 minutes.
Specifications
Diluent
Anhydrous Acetonitrile
Storage
Storage: Freezer storage, -10 to -30°C, dry
Stability
1-2 Days
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
