Biotin is a popular oligonucleotide modification and is used in a wide range of applications, including enrichment, detection, and immobilization assays. 5’-Biotin II consists of an ethylene glycol linker, an alternative structure to our traditional version. 5’-Biotin II phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques.
Coupling: 2 minute coupling time recommended
Deprotection: 5'-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step. If the final DMT-group is left on to aid in cartridge purification, we recommend that the oligo is left in contact with the TFA solution for 10 minutes.
Storage: Freezer storage, -10 to -30°C, dry
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.