Biotin-dT

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10-1038
5'-Dimethoxytrityloxy-5-[N-((4-t-butylbenzoyl)-biotinyl)-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C69H89N8O12PS
M.W.:
1285.55
F.W.:
684.70
CAS Number:
198080-40-9
Catalog Number: Select Pack Size and Format to Reveal

Description

Biotin-dT can replace dT residues within the oligonucleotide sequence. 5'-Biotin phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5'-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3' terminus. Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.

Details

Usage

  • Coupling: 15 minute coupling recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1038-02 0.25 g .25grams 1.94 51.33 30.8 19.25 14 10.27 2.57
10-1038-90 100 µmol .129grams 1 20 12 7.5 5.45 4 1
10-1038-95 50 µmol .064grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1038-02 0.25 g .25grams 2.9 0.07 51.6 32.25 23.45 3.23
10-1038-90 100 µmol .129grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1038-95 50 µmol .064grams 0.75 0.07 8.6 5.38 3.91 0.54


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