Azidobutyrate NHS Ester

default
50-1904
4-Azido-butan-1-oic acid N-Hydroxysuccinimide Ester

Product Specifications

Formula:
C8H10N4O4
M.W.:
226.19
F.W.:
113.12
CAS Number:
943858-70-6
Catalog Number: Select Pack Size and Format to Reveal

Description

Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence.

Details

Usage

  • Coupling: For labeling 0.2 umoles of an amino-modified oligonucleotide, dissolve 2.3 mg of Azidobutyrate NHS Ester in 60 uL of ACN (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500 uL of 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12 uL of the Azidobutyrate NHS Ester solution to the dissolved oligonucleotide and let react for 2 hours at room temperature and desalt.
  • Deprotection: Not Applicable
Specifications
Diluent Anhydrous ACN, DMSO or DMF
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


References

REFERENCES|(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.