Ultramild DNA Synthesis

An alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.

We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix. Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.

7 Product Results
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  1. Category UltraMild Base Protection Phosphoramidites and Supports Remove This Item
  • Ac-dC-CE Phosphoramidite
    Ac-dC-CE Phosphoramidite (10-1015)
    Catalog #: 10-1015  |  CAS #: 154110-40-4
    Formal Name: 5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • Pac-dA-CE Phosphoramidite
    Pac-dA-CE Phosphoramidite (10-1601)
    Catalog #: 10-1601  |  CAS #: 110543-74-3
    Formal Name:


  • iPr-Pac-dG-CE Phosphoramidite
    iPr-Pac-dG-CE Phosphoramidite (10-1621)
    Catalog #: 10-1621  |  CAS #: 150065-82-0
    Formal Name:


  • Ac-dC-CPG 500
    Ac-dC-CPG 500 (20-2013)
    Catalog #: 20-2013
    Formal Name: 5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 500
  • Ac-dC-CPG 1000
    Ac-dC-CPG 1000 (20-2015)
    Catalog #: 20-2015
    Formal Name: 5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 1000
  • Pac-dA-CPG
    Pac-dA-CPG (20-2601)
    Catalog #: 20-2601
    Formal Name: 5'-Dimethoxytrityl-N-phenoxyacetyl-2'-deoxyAdenosine,3'-succinoyl-long chain alkylamino-CPG
  • iPr-Pac-dG-CPG
    iPr-Pac-dG-CPG (20-2621)
    Catalog #: 20-2621
    Formal Name: 5'-Dimethoxytrityl-N-p-isopropyl-phenoxyacetyl-2'-deoxyGuanosine, 3'-succinoyl-long chain alkylamino-CPG
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