Chain Terminators

In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

2 Product Results
 
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  • 3'-dA-CPG
    3'-dA-CPG (20-2004)
    Catalog #: 20-2004
    Formal Name: 5'-Dimethoxytrityl-N-benzoyl-3'-deoxyAdenosine, 2'-succinoyl-long chain alkylamino-CPG
  • 3'-dC-CPG
    3'-dC-CPG (20-2064)
    Catalog #: 20-2064
    Formal Name: 5'-Dimethoxytrityl-N-benzoyl-3'-deoxyCytosine, 2'-succinoyl-long chain alkylamino-CPG 500
 
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