Selectively Binding Complementary (SBC) oligos

SBC oligos exhibit high affinity for natural oligonucleotides but they show little affinity for other SBC oligos even of a complementary sequence.  Oligos in which A has been replaced with 2-amino-A and T with 2-thio-T represent an excellent example of SBC oligos.  While 2-amino-A forms a very stable base pair with T containing three hydrogen bonds, the stability of the base pair with 2-thio-T is greatly diminished. However, 2-thio-T base pairs perfectly well with A. As an example, SBC 20mers annealed against a DNA 20mer target exhibited Tm values 10 °C higher than the corresponding DNA-DNA hybrid, whereas the SBC-SBC hybrid yielded Tm values 30 °C lower.

2 Product Results
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  • Pac-2-Amino-dA-CE Phosphoramidite
    Pac-2-Amino-dA-CE Phosphoramidite (10-1585)
    Catalog #: 10-1585
    Formal Name: 5'-Dimethoxytrityl-N2-phenoxyacetyl-N6-di-n-butylaminomethylidene-2,6-diamino-2'-deoxypurine riboside-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • 2-Thio-dT-CE Phosphoramidite
    2-Thio-dT-CE Phosphoramidite (10-1036)
    Catalog #: 10-1036  |  CAS #: 156783-23-2
    Formal Name: 5'-Dimethoxytrityl-(N3/O4-toluoyl)-2'-deoxy-2-thioThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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