Coupling: Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
Deprotection: See TB_Sulfurizing II.pdf
Specifications
Diluent
60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)