Sulfurizing Reagent II

Sulfurizing Reagent II
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Product Specifications

Formula:
C5H7N3S3
M.W.:
205.31
CAS Number:
1192027-04-5
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Technical Documents

Sulfurizing Reagent !! - Stable in Solution and Optimized for RNA Sulfurizaion

Safety Data Sheet

Glen Report 18.13: Sulfurizing Reagent II - Stable in Solution and Optimized for RNA Sulfurization

Glen Report 6.23: Research Review - Antisense RNA

Glen Report 18-1 Supplement: Sulfurizing Reagent II and its use in Synthesizing Oligonucleotide Phosphorothioates

Glen Report 4-16: Sulfurization of Oligonucleotides

Glen Report 32-27: Technical Snippets

Description

Glen Report 18.1: Sulfurizing Reagent Ii - Stable In Solution And Optimized For Rna Sulfurization

Glen Report 18.1 Supplement: Sulfurizing Reagent Ii And Its Use In Synthesizing Oligonucleotide Phosphorothioates

Details

Usage

  • Coupling: Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
  • Deprotection: See TB_Sulfurizing II.pdf
Specifications
Diluent 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
Storage Controlled room temperature or lower, dry
Stability At least 6 months

References

New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Förster resonance energy transfer probes in 5'-nuclease assays. Igor V. Kutyavin

Highly specific unnatural base pair systems as a third base pair for PCR amplification Rie Yamashige, Michiko Kimoto, Yusuke Takezawa, Akira Sato, Tsuneo Mitsui, Shigeyuki Yokoyama, and Ichiro Hirao

Mercury(II)-Catalyzed Cleavage, Isomerization and Depurination of RNA and DNA Model Compounds and Desulfurization of Their Phosphoromonothioate Analogs Saleh LY, Ora M, Lönnberg T.

Interaction of ASOs with PC4 Is Highly Influenced by the Cellular Environment and ASO Chemistry Vickers, T. A., Migawa, M. T., Seth, P. P., & Crooke, S. T.

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