Glen Research offers a range of different 5’-Amino-Modifiers designed for use in automated synthesizers to functionalize the 5’-terminus of a target oligonucleotide with a primary amine. These modifiers vary based on the amine protecting group and the linker. Of these, the trifluoroacetyl (TFA)-protected Amino-Modifiers are typically used when a trityl group is not necessary. The TFA group allows deprotection to be completed in a single standard step to give an amine that is immediately available for conjugation with amine-reactive agents.
The 5’-Amino-Modifier TEG has a 12-atom hydrophilic triethylene glycol linker with a TFA protecting group.
It should be noted that TFA-amino modifiers are sensitive to prolonged transit times and elevated temperatures (e.g., international shipments in the summer). Under these conditions, PDA-amino modifiers are significantly more stable and are excellent alternatives (https://www.glenresearch.com/reports/gr25-16).
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30°C, dry |
| Stability | 2-3 days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1917-02 | 0.25 g | .25grams | 5.11 | 157 | 94.2 | 58.88 | 42.82 | 31.4 | 7.85 |
| 10-1917-90 | 100 µmol | .049grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1917-02 | 0.25 g | .25grams | 7.62 | 0.07 | 146 | 91.25 | 66.36 | 9.13 |
| 10-1917-90 | 100 µmol | .049grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
