5'-Dimethoxytrityl-N2-[6-(trifluoroacetylamino)-hex-1-yl]-2'-deoxyGuanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.
Specifications | |
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Diluent | Anhydrous Acetonitrile |
Storage | Refrigerated storage, maximum of 2-8°C, dry |
Stability | 2-3 days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
---|---|---|---|---|---|---|---|---|---|
LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
10-1529-02 | 0.25 g | .25grams | 2.59 | 73 | 43.8 | 27.38 | 19.91 | 14.6 | 3.65 |
10-1529-90 | 100 µmol | .097grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-1529-95 | 50 µmol | .048grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
---|---|---|---|---|---|---|---|---|
Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
10-1529-02 | 0.25 g | .25grams | 3.87 | 0.07 | 71 | 44.38 | 32.27 | 4.44 |
10-1529-90 | 100 µmol | .097grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
10-1529-95 | 50 µmol | .048grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |