Amino-Modifier C6-U Phosphoramidite

5'-Dimethoxytrityl-5-[N-(trifluoroacetylaminohexyl)-3-acrylimido]-Uridine, 2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C60H84F3N6O12PSi
M.W.:
1197.41
F.W.:
474.40
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Amino-Modifier C6-U has been added to the growing family of sequence modifiers and we envisage applications in RNA structural studies as well as for labelling siRNA to probe uptake and cellular distribution.

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3039-02 0.25 g .25grams 2.09 56.33 33.8 21.13 15.36 11.27 2.82
10-3039-90 100 µmol .12grams 1 20 12 7.5 5.45 4 1
10-3039-95 50 µmol .06grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3039-02 0.25 g .25grams 3.12 0.07 56 35 11.67 3.5
10-3039-90 100 µmol .12grams 1.5 0.07 26.6 14.7 4.92 1.4
10-3039-95 50 µmol .06grams 0.75 0.07 8.6 5.3 1.79 0.5