Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5’ terminus or within the sequence, and a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group.
Coupling: Labeling: For labeling 0.2µmoles of an amino-modified oligonucleotide, dissolve 2.3mg of the Alkyne-Modifier NHS Ester in 60µL of Acetonitrile (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500µL 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12µL of the Alkyne-Modifier NHS Ester solution and vortex. Let react for 2 hours at room temperature and desalt.
Deprotection: Not Applicable
Freezer storage, -10 to -30°C, dry
REFERENCES|(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.|(2) J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.