Alkyne-Modifier Serinol Phosphoramidite

3-Dimethoxytrityloxy-2-(3-(5-hexynamido)propanamido)propyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C42H55N4O7P
M.W.:
758.88
F.W.:
318.26
CAS Number:
2084118-98-7
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5’ terminus or within the sequence, and a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group.

Details

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours|at 65°C, 17 hours at room temperature or AMA (ammonium hydroxide/40% methylamine 1:1) for 10 minutes at 65°C.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days

Intellectual Property

This product is covered by US Patent No.: 8,394,948.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1992-02 0.25 g .25grams 3.29 96.33 57.8 36.13 26.27 19.27 4.82
10-1992-90 100 µmol .076grams 1 20 12 7.5 5.45 4 1
10-1992-95 50 µmol .038grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1992-02 0.25 g .25grams 4.92 0.07 92 57.5 41.82 5.75
10-1992-90 100 µmol .076grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1992-95 50 µmol .038grams 0.75 0.07 8.6 5.38 3.91 0.54

References

REFERENCES|(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.|(2) J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.