Acridine Phosphoramidite

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10-1973
1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C51H60N4O6PCl
M.W.:
891.53
F.W.:
450.86
Catalog Number: Select Pack Size and Format to Reveal

Description

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3'-terminus. Acridine is an effective intercalating agent.

Details

Usage

  • Coupling: 15 minute coupling time is recommended.|(Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.)
  • Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.|Note: NaOH is not compatible with dmf protecting groups.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1973-02 0.25 g .25grams 2.8 80 48 30 21.82 16 4
10-1973-90 100 µmol .089grams 1 20 12 7.5 5.45 4 1
10-1973-95 50 µmol .045grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1973-02 0.25 g .25grams 4.19 0.07 77.4 48.38 35.18 4.84
10-1973-90 100 µmol .089grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1973-95 50 µmol .045grams 0.75 0.07 8.6 5.38 3.91 0.54


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