Acridine Phosphoramidite

1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C51H60N4O6PCl
M.W.:
891.53
F.W.:
450.86
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3'-terminus. Acridine is an effective intercalating agent.

Details

Usage

  • Coupling: 15 minute coupling time is recommended.|(Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.)
  • Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.|Note: NaOH is not compatible with dmf protecting groups.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1973-02 0.25 g .25grams 2.8 80 48 30 21.82 16 4
10-1973-90 100 µmol .089grams 1 20 12 7.5 5.45 4 1
10-1973-95 50 µmol .045grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1973-02 0.25 g .25grams 4.19 0.07 77.4 48.38 35.18 4.84
10-1973-90 100 µmol .089grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1973-95 50 µmol .045grams 0.75 0.07 8.6 5.38 3.91 0.54


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