Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C41H50N5O8P
M.W.:
771.85
F.W.:
289.18
CAS Number:
154110-40-4
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Similar to dA,C,G,T-CE Phosphoramidites


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1015-02 0.25 g .25grams 3.24 94.67 56.8 35.5 25.82 18.93 4.73
10-1015-05 0.5 g .5grams 6.48 202.67 121.6 76 55.27 40.53 10.13
10-1015-10 1.0 g 1grams 12.96 418.67 251.2 157 114.18 83.73 20.93
10-1015-20 2.0 g 2grams 25.91 850.33 510.2 318.88 231.91 170.07 42.52
10-1015-40 4.0 g 4grams 51.82 1714 1028.4 642.75 467.45 342.8 85.7

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1015-C2 0.25 g .25grams 4.83 0.07 93.4 58.38 42.45 5.84
10-1015-C5 0.5 g .5grams 9.67 0.07 190.2 118.88 86.45 11.89
10-1015-1C 1.0 g 1grams 19.34 0.07 383.6 239.75 174.36 23.98
10-1015-2C 2.0 g 2grams 38.67 0.07 770.2 481.38 350.09 48.14


Product FAQs

  • How does this work? What would happen if I used the AMA reagent with Bz protected dC?
  • Can the AMA reagent be made up and stored? for how long? in what kind of container?