A-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-Adenosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C52H72N7O9PSi
M.W.:
998.24
F.W.:
329.21
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3004-02 0.25 g .25grams 2.5 70 42 26.25 19.09 14 3.5
10-3004-05 0.5 g .5grams 5.01 153.67 92.2 57.63 41.91 30.73 7.68
10-3004-10 1.0 g 1grams 10.02 320.67 192.4 120.25 87.45 64.13 16.03

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3004-02 0.25 g .25grams 3.74 0.07 68 42 14.25 4
10-3004-05 0.5 g .5grams 7.48 0.07 143 89 29.83 8
10-3004-10 1.0 g 1grams 14.95 0.07 292 182 60.96 18