Vitamin E is both lipophilic and non-toxic even at high doses so would be an excellent candidate as a lipophilic carrier for oligonucleotides. Therefore, as an addition to our cholesteryl product line, we offer simple a-tocopheryl (vitamin E) labelling. Totally synthetic a-tocopherol is racemic at its three chiral centers and is used to prepare this product.
Coupling: 15 minute coupling time. DCI activator (30-3150-xx) is recommended. To maintain label yield, carry out the synthesis DMT-ON.
Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with ACN. At this point, the DMT group may be safely removed without loss of label during deprotection.