α-Tocopherol-TEG Phosphoramidite

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10-1977
1-Dimethoxytrityloxy-3-O-[(9-DL-α-tocopheryl)-triethyleneglycol-1-yl]-glyceryl-2-O-[(2-cyanoethyl)-(N,N,-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C68H103N2O10P
M.W.:
1139.56
F.W.:
698.91
Catalog Number: Select Pack Size and Format to Reveal

Description

Vitamin E is both lipophilic and non-toxic even at high doses so would be an excellent candidate as a lipophilic carrier for oligonucleotides. Therefore, as an addition to our cholesteryl product line, we offer simple a-tocopheryl (vitamin E) labelling. Totally synthetic a-tocopherol is racemic at its three chiral centers and is used to prepare this product.

Details

Usage

  • Coupling: 15 minute coupling time. DCI activator (30-3150-xx) is recommended. To maintain label yield, carry out the synthesis DMT-ON.
  • Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with ACN. At this point, the DMT group may be safely removed without loss of label during deprotection.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1977-02 0.25 g .25grams 2.19 59.67 35.8 22.38 16.27 11.93 2.98
10-1977-90 100 µmol .114grams 1 20 12 7.5 5.45 4 1
10-1977-95 50 µmol .057grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1977-02 0.25 g .25grams 3.27 0.07 59 36.88 26.82 3.69
10-1977-90 100 µmol .114grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1977-95 50 µmol .057grams 0.75 0.07 8.6 5.38 3.91 0.54