Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Mild Deprotection: Ammonium hydroxide for 24hours at room temperature.