8-Br-dG-CE Phosphoramidite

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10-1027
5'-Dimethoxytrityl-N2-dimethylaminomethylidene-8-bromo-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H52N8O7PBr
M.W.:
903.9
F.W.:
408.10
CAS Number:
198080-37-4
Catalog Number: Select Pack Size and Format to Reveal

Description

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Mild Deprotection: Ammonium hydroxide for 24hours at room temperature.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1027-02 0.25 g .25grams 2.77 79 47.4 29.63 21.55 15.8 3.95
10-1027-90 100 µmol .09grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1027-02 0.25 g .25grams 4.13 0.07 76.2 47.63 34.64 4.76
10-1027-90 100 µmol .09grams 1.5 0.07 23.6 14.75 10.73 1.48