8-Br-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N6-dimethylaminomethylidene-8-bromo-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H52N8O6PBr
M.W.:
887.81
F.W.:
392.11
CAS Number:
207906-54-5
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Mild Deprotection: Ammonium hydroxide for 24hours at room temperature.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1007-02 0.25 g .25grams 2.82 80.67 48.4 30.25 22 16.13 4.03
10-1007-90 100 µmol .089grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1007-02 0.25 g .25grams 4.2 0.07 77.6 48.5 35.27 4.85
10-1007-90 100 µmol .089grams 1.5 0.07 23.6 14.75 10.73 1.48