8-Aza-7-deaza-A-CE Phosphoramidite

5'-Dimethoxytrityl-N6-dimethylaminomethylidene-8-aza-7-deaza-Adenosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C49H67N8O7PSi
M.W.:
939.16
F.W.:
329.21
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

8-Aza-7-deaza-Adenosine is an isomer of Adenosine with virtually identical electron density. The N7 nitrogen is not available for hydrogen bonding.

Details

Usage

  • Coupling: 12 minute coupling time recommended.
  • Deprotection: RNA Deprotection-see bulletin.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3083-02 0.25 g .25grams 2.66 75.33 45.2 28.25 20.55 15.07 3.77
10-3083-90 100 µmol .094grams 1 20 12 7.5 5.45 4 1
10-3083-95 50 µmol .047grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3083-02 0.25 g .25grams 3.97 0.07 73 45.6 15.21 4.5
10-3083-90 100 µmol .094grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3083-95 50 µmol .047grams 0.75 0.07 8.6 5.3 1.79 0.5