8-Amino-G is formed along with 8-oxo-G as the major mutagenic lesions formed in DNA damage caused by 2-nitropropane. 2-Nitropropane is an industrial solvent and a component of paints, dyes and varnishes, and is also present in cigarette smoke.
8-Amino-dA and 8-amino-dG are useful in triplex formation due to the presence of the additional amino groups.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.1M 2-mercaptoethanol, (7µl/ml) at 55° for 20 hours.