8-Amino-dG-CE Phosphoramidite

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10-1079
5'-Dimethoxytrityl-N2,N8-bis(dimethylaminomethylidine)-8-amino-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H59N10O7P
M.W.:
895.01
F.W.:
344.22
CAS Number:
166092-91-7
Catalog Number: Select Pack Size and Format to Reveal

Description

8-Amino-G is formed along with 8-oxo-G as the major mutagenic lesions formed in DNA damage caused by 2-nitropropane. 2-Nitropropane is an industrial solvent and a component of paints, dyes and varnishes, and is also present in cigarette smoke. 8-Amino-dA and 8-amino-dG are useful in triplex formation due to the presence of the additional amino groups.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.1M 2-mercaptoethanol, (7µl/ml) at 55° for 20 hours.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1079-02 0.25 g .25grams 2.79 79.67 47.8 29.88 21.73 15.93 3.98
10-1079-90 100 µmol .09grams 1 20 12 7.5 5.45 4 1
10-1079-95 50 µmol .045grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1079-02 0.25 g .25grams 4.17 0.07 77 48.13 35 4.81
10-1079-90 100 µmol .09grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1079-95 50 µmol .045grams 0.75 0.07 8.6 5.38 3.91 0.54