8-Amino-dA-CE Phosphoramidite

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10-1086
5'-Dimethoxytrityl-N6,N8-bis(dimethylaminomethylidine)-8-amino-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H59N10O6P
M.W.:
879.01
F.W.:
328.22
CAS Number:
211676-21-0
Catalog Number: Select Pack Size and Format to Reveal

Description

8-Amino-dA and 8-amino-dG are useful in triplex formation due to the presence of the additional amino groups. 2'-DeoxyXanthosine (dX) is a naturally occurring nucleoside that may be derived from oxidative deamination of 2'-deoxyGuanosine (dG). dX has a similar bonding pattern to thymidine and it may base pair with dA, with such purine-purine interactions causing duplex distortion. dX also featured in attempts to extend the genetic alphabet with a new base pair of dX and pyrimidine-2,4-diamine nucleoside. dX has also interested researchers in the field of DNA damage and repair since it is a product of nitric oxide-induced mutagenesis.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1086-02 0.25 g .25grams 2.84 81.33 48.8 30.5 22.18 16.27 4.07
10-1086-90 100 µmol .088grams 1 20 12 7.5 5.45 4 1
10-1086-95 50 µmol .044grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1086-02 0.25 g .25grams 4.24 0.07 78.4 49 35.64 4.9
10-1086-90 100 µmol .088grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1086-95 50 µmol .044grams 0.75 0.07 8.6 5.38 3.91 0.54