6-Thio-G-CE Phosphoramidite

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10-3072
5'-Dimethoxytrityl-N2-isobutyryl-6-(2-cyanoethyl)thio-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C53H71N8O8PSSi
M.W.:
1039.31
F.W.:
361.26
Catalog Number: Select Pack Size and Format to Reveal

Description

6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used.

Details

Usage

  • Coupling: 12 minute coupling time recommended. Use 0.02M Iodine to avoid degradation and Ac-C (10-3015).
  • Deprotection: Treat column with 1M DBU in ACN for 4 hrs at RT, rinse and dry support. Then deprotect with 50mM NaSH in AMA at 65°C for 10 minutes. Complete using RNA deprotection. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3072-02 0.25 g .25grams 2.41 67 40.2 25.13 18.27 13.4 3.35
10-3072-90 100 µmol .104grams 1 20 12 7.5 5.45 4 1
10-3072-95 50 µmol .052grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3072-02 0.25 g .25grams 3.59 0.07 0 0 13.63 0
10-3072-90 100 µmol .104grams 1.5 0.07 0 0 4.92 0
10-3072-95 50 µmol .052grams 0.75 0.07 0 0 1.79 0

References

1 C.J. Adams, J.B. Murray, M.A. Farrow, J.R.P. Arnold, and P.G. Stockley, Tetrahedron Lett., 1995, 36, 5421-5424.
2 D.A. Berry, et al., Tetrahedron Lett, 2004, 45, 2457-2461.