ROX NHS Ester

6-Carboxy-X-rhodamine, N-hydroxysuccinimide ester

Product Specifications

Formula:
C37H33N3O7
M.W.:
631.69
F.W.:
516.60
CAS Number:
1890922-83-4
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Description

ROX is a rhodamine dye that has played a significant role in dideoxy Sanger sequencing alongside FAM, JOE and TAMRA. The dye itself is also commonly used for qPCR applications as a passive reference dye. ROX is not available as a phosphoramidite, and as such, the use of this NHS ester is the standard method of incorporating ROX into oligonucleotides. This NHS ester is offered as the 6-isomer.

Details

Usage

  • Coupling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 3.2 mg of the ROX NHS Ester in 100 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react for 60 min at room temperature and desalt.
Specifications
Diluent DMF or DMSO
Storage Freezer storage, -10 to -30°C, dry