Thiazole Orange NHS Ester

N-(5-carboxypentyl)-2-[(1,4-dihydro-1- methylquinolin-4-ylidene)methyl]benzothiazol-3-ium chloride N-hydroxysuccinimide ester

Product Specifications

Formula:
C28H28ClN3O4S
M.W.:
538.06
F.W.:
386.51
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Description

Thiazole orange is an asymmetric cyanine dye whose fluorescence can be quite dependent on its local environment. When an oligonucleotide labeled with thiazole orange is hybridized to its complementary sequence, the thiazole orange acts as an intercalator. In addition to providing enhanced thermal stability, the dye adopts a mostly planar configuration resulting in significantly enhanced fluorescence. This “light up” effect can be as high as 34-fold depending on the sequence and how the dye is attached. This NHS ester will allow simple functionalization of internally located amino modifications such as those generated with amino-modifier C6 dT (10-1039).

Details

Usage

  • Coupling: Labeling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 5.4 mg of the Thiazole Orange NHS Ester in 200 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the Thiazole Orange NHS Ester solution and vortex. Let react for 15 min at room temperature and desalt.
Specifications
Diluent DMF or DMSO
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days