5-Me-C-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-5-methyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C52H74N5O10PSi
M.W.:
988.25
F.W.:
319.21
CAS Number:
872548-65-7
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Details

Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3064-02 0.25 g .25grams 2.53 71 42.6 26.63 19.36 14.2 3.55
10-3064-90 100 µmol .099grams 1 20 12 7.5 5.45 4 1
10-3064-95 50 µmol .049grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3064-02 0.25 g .25grams 3.78 0.07 69.2 43.2 14.42 4.3
10-3064-90 100 µmol .099grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3064-95 50 µmol .049grams 0.75 0.07 8.6 5.3 1.79 0.5