5-Me-2'-deoxyZebularine-CE Phosphoramidite

default
10-1061
5'-Dimethoxytrityl-5-methyl-pyrimidin-2-one-2'-deoxyriboside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C40H49N4O7P
M.W.:
728.82
F.W.:
288.19
CAS Number:
125258-62-0
Catalog: Make Selections to Reveal

Description

Zebularine (pyrimidin-2-one ribonucleoside) is a cytidine analogue that acts as a DNA demethylase inhibitor, as well as a cytidine deaminase inhibitor. This structure is very active biologically and Zebularine is now used as a potent anti-cancer drug. A 2'-deoxynucleoside analogue of Zebularine, 5-methyl-pyrimidin-2-one, 2'-deoxynucleoside, has been used to probe the initiation of the cellular DNA repair process by making use of its mildly fluorescent properties. This combination of biological activity and fluorescence properties would make 5-Me-2'-deoxyZebularine a strong addition to our array of nucleoside analogues.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Ammonium Hydroxide for 24 hrs at room temperature or as required by nucleobases.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1061-02 0.25 g .25grams 3.43 101 60.6 37.88 27.55 20.2 5.05
10-1061-90 100 µmol .073grams 1 20 12 7.5 5.45 4 1
10-1061-95 50 µmol .036grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1061-02 0.25 g .25grams 5.12 0.07 96 60 43.64 6
10-1061-90 100 µmol .073grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1061-95 50 µmol .036grams 0.75 0.07 8.6 5.38 3.91 0.54