5'-Maleimide-Modifier Phosphoramidite

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10-1938

2-(1,7-Dimethyl-3,5-dioxo-10-oxa-4-azatricyclo[5.2.1.02,6]dec-8-en-4-yl)-ethyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C21H32N3O5P
M.W.:
437.47
F.W.:
299.22 (pre-retro-Diels-Alder)| FW: 203.09 (maleimide)
Catalog Number: Select Pack Size and Format to Reveal

Description

5’-Maleimide Modifier Phosphoramidite, developed at the University of Barcelona, incorporates a maleimide cycloadduct that is stable to ammonium hydroxide at room temperature. This phosphoramidite can be incorporated into DNA and RNA with both phosphate and phosphorothioate linkages. A retro–Diels-Alder reaction deprotects the maleimide immediately prior to conjugation.

Details

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/Maleimide_Modifier.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days

Intellectual Property

5'-Maleimide Modifier Phosphoramidite is protected by a patent application and is offered by Glen Research under a license agreement from the University of Barcelona.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1938-02 0.25 g .25grams 5.71 177 106.2 66.38 48.27 35.4 8.85
10-1938-90 100 µmol .044grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1938-02 0.25 g .25grams 8.53 0.07 164.2 102.63 74.64 10.26
10-1938-90 100 µmol .044grams 1.5 0.07 23.6 14.75 10.73 1.48