5-I-U-CE Phosphoramidite

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10-3091
5'-Dimethoxytrityl-5-iodo-2'-Uridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C45H60N4O9PISi
M.W.:
986.96
F.W.:
432.07
Catalog Number: Select Pack Size and Format to Reveal

Description

5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments.

Details

Usage

  • Coupling: 12 minute coupling time
  • Deprotection: Ammonium hydroxide/Ethanol 3:1 (v/v) at room temperature as required by the nucleobases. Complete the 2' deprotection using standard RNA protocols. See http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3091-02 0.25 g .25grams 2.53 71 42.6 26.63 19.36 14.2 3.55
10-3091-90 100 µmol .099grams 1 20 12 7.5 5.45 4 1
10-3091-95 50 µmol .049grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3091-02 0.25 g .25grams 3.78 0.07 69.2 43.2 14.42 4.3
10-3091-90 100 µmol .099grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3091-95 50 µmol .049grams 0.75 0.07 8.6 5.3 1.79 0.5