5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments.
Coupling: 12 minute coupling time
Deprotection: Ammonium hydroxide/Ethanol 3:1 (v/v) at room temperature as required by the nucleobases. Complete the 2' deprotection using standard RNA protocols. See http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf.
Freezer storage, -10 to -30°C, dry
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.